Glucan, water dikinase phosphorylates crystalline maltodextrins and thereby initiates solubilization
نویسندگان
چکیده
منابع مشابه
The two plastidial starch-related dikinases sequentially phosphorylate glucosyl residues at the surface of both the A- and B-type allomorphs of crystallized maltodextrins but the mode of action differs.
In this study, two crystallized maltodextrins were generated that consist of the same oligoglucan pattern but differ strikingly in the physical order of double helices. As revealed by x-ray diffraction, they represent the highly ordered A- and B-type allomorphs. Both crystallized maltodextrins were similar in size distribution and birefringence. They were used as model substrates to study the c...
متن کاملRunning head: Phosphorylation of A- and B-type crystallized maltodextrins
138 In this study, two crystallized maltodextrins were generated that consist of the same 139 oligoglucan pattern but differ strikingly in the physical order of double helices. As revealed 140 by X-ray diffraction, they represent the highly ordered Aor B-type allomorph. Both 141 crystallized maltodextrins were similar in size distribution and birefringence. They were used 142 as model substrate...
متن کاملThe starch-related R1 protein is an -glucan, water dikinase
To determine the enzymatic function of the starch-related R1 protein it was heterologously expressed in Escherichia coli and purified to apparent homogeneity. Incubation of the purified protein with various phosphate donor and acceptor molecules showed that R1 is capable of phosphorylating glucosyl residues of -glucans at both the C-6 and the C-3 positions in a ratio similar to that occurring n...
متن کاملAn extra-plastidial a-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation
Starch phosphorylation catalysed by the a-glucan, water dikinases (GWD) has profound effects on starch degradation in plants. The Arabidopsis thaliana genome encodes three isoforms of GWD, two of which are localized in the chloroplast and are involved in the degradation of transient starch. The third isoform, termed AtGWD2 (At4g24450), was heterologously expressed and purified and shown to have...
متن کاملAn extra-plastidial alpha-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation.
Starch phosphorylation catalysed by the alpha-glucan, water dikinases (GWD) has profound effects on starch degradation in plants. The Arabidopsis thaliana genome encodes three isoforms of GWD, two of which are localized in the chloroplast and are involved in the degradation of transient starch. The third isoform, termed AtGWD2 (At4g24450), was heterologously expressed and purified and shown to ...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2008
ISSN: 0960-7412,1365-313X
DOI: 10.1111/j.1365-313x.2008.03513.x